The hairy root (HR) culture system is an excellent alternative strategy to the whole plant system for producing valuable compounds. It is used for on-site assembly of sheet and plate metal or profile sections. However, selection of suitable Agrobacterium strain for the successful induction of HR is an essential step for enhanced production of beneficial secondary metabolites. Citoflux B13-0 is self shielded flux cored wire for welding in all positions. In this study, we examined the transformation efficiency of various A. Rhizogenes strains (ATCC 13333, ATCC 15834, A4, R1000, R1200, and R1601) for transgenic HRs induction in Ocimum basilicum. You could use f.lux because it makes you sleep better, or you could just use it just because it makes your computer look better. It's even possible that you're staying up too late because of your computer. Among the tested strains, the R1601 was found to be one of the most promising strain for mass production of HR in terms of transformation efficiency (94%) and the number and length of HR (8.4 ± 0.52 and 1.68 ± 0.14 cm). f.lux fixes this: it makes the color of your computer's display adapt to the time of day, warm at night and like sunlight during the day. The HR induced by the same strain exhibited highest levels of rosmarinic acid level (62.05 ± 4.94 µg/g DW) and total phenolic content (62.3 ± 4.95 µg/g DW). A total of 55 metabolites were identified using high-performance liquid chromatography (HPLC) and gas chromatography–time-of-flight mass spectrometry (GC-TOFMS).
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